CRISPR-Cas type III-D effector complex
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8s9t
Q-score: 0.623
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a target RNA
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8s9u
Q-score: 0.584
Schwartz EA, Bravo JPK, Ahsan F, Macias LA, Brodbelt JS, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
To Be Published
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex bound to target rna (351 kDa, Complex from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8s9v
Q-score: 0.561
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to self-target RNA in a post-cleavage state
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8s9x
Q-score: 0.456
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex consensus map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex local refinement map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Crispr-cas type iii-d effector complex (351 kDa, Complex from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a target RNA local refinement map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
CRISPR-Cas type III-D effector complex bound to a target RNA consensus map
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
Bottom cylinder of high-resolution phycobilisome quenched by OCP (local refinement)
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8to2
Q-score: 0.592
Sauer PV, Cupellini L, Sutter M, Bondanza M, Dominguez Martin MA, Kirst H, Bina D, Koh AF, Kotecha A, Greber BJ, Nogales E, Polivka T, Mennucci B, Kerfeld CA
Sci Adv (2024) 10 pp. eadk7535-eadk7535 [ Pubmed: 38578996 DOI: doi:10.1126/sciadv.adk7535 ]
- Allophycocyanin beta chain (17 kDa, Protein from Synechocystis sp. PCC 6803)
- Translation initiation factor if-2 (12 kDa, Protein from Synechocystis sp. PCC 6803)
- Beta,beta-carotene-4,4'-dione (564 Da, Ligand)
- Phycocyanobilin (588 Da, Ligand)
- Phycobilisome 7.8 kda linker polypeptide, allophycocyanin-associated, core (7 kDa, Protein from Synechocystis sp. PCC 6803)
- Allophycocyanin subunit beta-18 (18 kDa, Protein from Synechocystis sp. PCC 6803)
- Allophycocyanin subunit alpha-b (17 kDa, Protein from Synechocystis sp. PCC 6803)
- Complex of phycobilisome from synechocystis pcc 6803 bound to ocp (Complex from Synechocystis sp. (strain PCC 6803 / Kazusa))
- Allophycocyanin alpha chain (17 kDa, Protein from Synechocystis sp. PCC 6803)
- Phycobiliprotein apce (100 kDa, Protein from Synechocystis sp. PCC 6803)
- Phycobilisome rod-core linker polypeptide cpcg (28 kDa, Protein from Synechocystis sp. PCC 6803)
- Orange carotenoid-binding protein (34 kDa, Protein from Synechocystis sp. PCC 6803)
Central rod disk in C1 symmetry of high-resolution phycobilisome quenched by OCP (local refinement)
Resolution: 1.87 Å
EM Method: Single-particle
Fitted PDBs: 8to5
Q-score: 0.833
Sauer PV, Cupellini L, Sutter M, Bondanza M, Dominguez Martin MA, Kirst H, Bina D, Koh AF, Kotecha A, Greber BJ, Nogales E, Polivka T, Mennucci B, Kerfeld CA
Sci Adv (2024) 10 pp. eadk7535-eadk7535 [ Pubmed: 38578996 DOI: doi:10.1126/sciadv.adk7535 ]
- Phycobilisome 32.1 kda linker polypeptide, phycocyanin-associated, rod 1 (32 kDa, Protein from Synechocystis sp. PCC 6803)
- C-phycocyanin alpha subunit (17 kDa, Protein from Synechocystis sp. PCC 6803)
- Phycobilisome 32.1 kda linker polypeptide, phycocyanin-associated, rod 2 (30 kDa, Protein from Synechocystis sp. PCC 6803)
- Phycocyanobilin (588 Da, Ligand)
- Water (18 Da, Ligand)
- Complex of phycobilisome from synechocystis pcc 6803 bound to ocp (Complex from Synechocystis sp. (strain PCC 6803 / Kazusa))
- C-phycocyanin beta subunit (18 kDa, Protein from Synechocystis sp. PCC 6803)